Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
نویسندگان
چکیده
منابع مشابه
Protein quantitation using mass spectrometry.
Mass spectrometry is a method of choice for quantifying low-abundance proteins and peptides in many biological studies. Here, we describe a range of computational aspects of protein and peptide quantitation, including methods for finding and integrating mass spectrometric peptide peaks, and detecting interference to obtain a robust measure of the amount of proteins present in samples.
متن کاملDetermination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry.
Micro-quantities of protein-derived peptides have been converted into N-acetylated permethyl derivatives, and their sequences determined by low-resolution mass spectrometry without prior knowledge of their amino acid compositions or lengths. A new strategy is suggested for the mass spectrometric sequencing of oligopeptides or proteins, involving gel filtration of protein hydrolysates and subseq...
متن کاملComparison of Two Multiplex PCR Systems for Meat Species Authentication
Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication. Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...
متن کاملMass Spectrometry: Quantitation
graphy (LC) has been used for identification of proteins excised from 1-D or 2-D gels. The automated nature of protein database searching using uninterpreted MS/MS data (4), the low femtomole sensitivity and the ability to identify a protein based on a single MS/MS product ion spectrum allow the identification of more than 100 proteins per day. Thus, extensive protein identifications have been ...
متن کاملPrimary Structure Determination of Peptides and Proteins by Mass Spectrometry
A pressing problem in modern organic chemistry is the determination of the structure of polypeptides and proteins, for it is here that the needs and interests of biochemistry have far surpassed present chemical possibilities, while elucidation of many a biological problem on the molecular level requires a clear understanding of the relation between structure and biological function. I should li...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2016
ISSN: 1940-087X
DOI: 10.3791/54420